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KMID : 1157520110070020077
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International Journal of Clinical Preventive Dentistry
2011 Volume.7 No. 2 p.77 ~ p.79
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Effect of Dentifrice Containing Enzyme on Mutans of Streptococcus mutans in Plaque in Orthodontic Patients
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Sarworini Bagio Budiardjo
Harun Asyiq Gunawan
Soeherwin Mangundjaja
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Abstract
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Objective: Dentifrice containing enzyme of Amyloglucosidase (Am) and Glucoseoxidase (GO) 1.5% and Sodium fluoride 0.24% has a broad bacteriostatic agent and is generally more effective against Gram-positive than against Gram-negative bacteria. The aim of the study is to determine the effect of dentifrice containing enzyme in inhibiting the growth of mutans of Streptococcus mutans in plaque.
Methods: Before enrolled in the study, respondents fill and signature the informed consent. Ten respondents wearing fixed orthodontic appliances part icipated as the subjects, conducting two times of treatment as follows: ten as treatment group before and after tooth brushing with dentifrice containing enzyme, whereas ten subjects as control group before and after tooth brushing with dentifrice containing non-enzyme. Plaque samples were collected before and after tooth brushing with dentifrice containing enzyme and with non-enzyme, A serial dilution was made, followed by inoculating on TYS20B medium (Schaeken, M.J.M, et al,1986). Data which were obtained from Colony Forming Units of mutans of Streptococcus mutans grew on the TYS20B medium before and after tooth brushing were analyzed in a descriptive and "t" test.
Results: Results showed that there was no significant difference in the average amount of Streptococcus mutans colonies between before and after using non-enzyme. However, a significant difference (p=0.000) was found respectively as a result of before and after tooth brushing with dentifrice containing enzyme.
Conclusion: Tooth brushing with dentifrice containing enzyme can inhibit the growth of mutans of Streptococcus mutans in plaque. It can be used in preventing caries risk in orthodontic patients.
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KEYWORD
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dentifrices, enzymes, Streptococcus mutans, orthodontics
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